The generation of an infinite number of customized cells is made possible by the EBV-based immortalization technique, which is quick, dependable, and efficient. Therefore, EBV immortalized B cell lines constitute a theoretically infinite source of genomic DNA, expression RNA, protein, and system biology research and are widely employed as a source of biological material for functional and molecular studies. Furthermore, these cell lines can be employed as a culture model for a range of investigations, such as high throughput biological studies, large-scale drug library screening, functional and serological studies, molecular and genetic research, and vaccine studies.

In stem cell research, B cell immortalization holds significant potential, especially for the production of induced pluripotent stem cells (iPSCs). A nearly limitless supply of cells that can be used for iPSC reprogramming can be obtained through B cell immortalization. Furthermore, B cells are a commonly available source of cells for iPSC production since they are simple to extract from peripheral blood or other organs.